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Addgene inc larry barcode library
a. The expression dynamics of perturbed genes during hPSC-derived islet differentiation, based on in-house bulk RNA-seq. b. The list of perturbed genes, categorized by the function, mutant type, cell background and clone number in the knockout village. c. Left: quality control strategy on <t>LARRY</t> <t>barcode</t> libraries and transcriptome libraries. Right: The fraction of cells passing both filtering steps. d. Histogram showing the distribution of LARRY barcode read counts before filtering steps. e. Stacked line plot showing the clone representation during differentiation. Each line represents a clone. f. Cell numbers of individual clones during differentiation. The lines represent the median cell number. g. Histograms showing the contribution of read depth, cell background, and genotype to gene expression variance. h. Dot plot showing the expression of marker genes associated with each cell type. i. UMAP embedding of scRNA-seq dataset, annotated by cell cycle phase.
Larry Barcode Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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larry barcode library - by Bioz Stars, 2026-04
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a. The expression dynamics of perturbed genes during hPSC-derived islet differentiation, based on in-house bulk RNA-seq. b. The list of perturbed genes, categorized by the function, mutant type, cell background and clone number in the knockout village. c. Left: quality control strategy on LARRY barcode libraries and transcriptome libraries. Right: The fraction of cells passing both filtering steps. d. Histogram showing the distribution of LARRY barcode read counts before filtering steps. e. Stacked line plot showing the clone representation during differentiation. Each line represents a clone. f. Cell numbers of individual clones during differentiation. The lines represent the median cell number. g. Histograms showing the contribution of read depth, cell background, and genotype to gene expression variance. h. Dot plot showing the expression of marker genes associated with each cell type. i. UMAP embedding of scRNA-seq dataset, annotated by cell cycle phase.

Journal: bioRxiv

Article Title: A stem cell knockout village reveals lineage rewiring and a non-canonical islet cell fate in monogenic diabetes

doi: 10.64898/2025.12.23.696311

Figure Lengend Snippet: a. The expression dynamics of perturbed genes during hPSC-derived islet differentiation, based on in-house bulk RNA-seq. b. The list of perturbed genes, categorized by the function, mutant type, cell background and clone number in the knockout village. c. Left: quality control strategy on LARRY barcode libraries and transcriptome libraries. Right: The fraction of cells passing both filtering steps. d. Histogram showing the distribution of LARRY barcode read counts before filtering steps. e. Stacked line plot showing the clone representation during differentiation. Each line represents a clone. f. Cell numbers of individual clones during differentiation. The lines represent the median cell number. g. Histograms showing the contribution of read depth, cell background, and genotype to gene expression variance. h. Dot plot showing the expression of marker genes associated with each cell type. i. UMAP embedding of scRNA-seq dataset, annotated by cell cycle phase.

Article Snippet: Individual GFP-barcode plasmids were cloned from the LARRY barcode library (Addgene #140024) and then transfected to 293T cells to produce lentivirus.

Techniques: Expressing, Derivative Assay, RNA Sequencing, Mutagenesis, Knock-Out, Control, Clone Assay, Gene Expression, Marker